diff --git a/biology/seqkit/Makefile b/biology/seqkit/Makefile
index a58c6f20115a..2ddb5ee645d8 100644
--- a/biology/seqkit/Makefile
+++ b/biology/seqkit/Makefile
@@ -1,7 +1,6 @@
 PORTNAME=	seqkit
 DISTVERSIONPREFIX=	v
-DISTVERSION=	2.10.0
-PORTREVISION=	9
+DISTVERSION=	2.10.1
 CATEGORIES=	biology
 
 MAINTAINER=	yuri@FreeBSD.org
diff --git a/biology/seqkit/distinfo b/biology/seqkit/distinfo
index 15f7c568f012..04dc98eed337 100644
--- a/biology/seqkit/distinfo
+++ b/biology/seqkit/distinfo
@@ -1,7 +1,7 @@
-TIMESTAMP = 1741844878
-SHA256 (go/biology_seqkit/seqkit-v2.10.0/v2.10.0.mod) = 1c6d38c7d0f78167b5b058b257703084f184e420c2aa7750e0881e4e974ade28
-SIZE (go/biology_seqkit/seqkit-v2.10.0/v2.10.0.mod) = 2514
-SHA256 (go/biology_seqkit/seqkit-v2.10.0/v2.10.0.zip) = ca5ef994e7f02ed4e40efefb3dd32833c335c8920fd369af3e44167421609b05
-SIZE (go/biology_seqkit/seqkit-v2.10.0/v2.10.0.zip) = 34094634
-SHA256 (go/biology_seqkit/seqkit-v2.10.0/golang-sys-v0.6.0_GH0.tar.gz) = b4f6d17c7a128f76169964b437cb66b3f2dbf9a33361928ec19dfecf7b03fc54
-SIZE (go/biology_seqkit/seqkit-v2.10.0/golang-sys-v0.6.0_GH0.tar.gz) = 1434234
+TIMESTAMP = 1762963353
+SHA256 (go/biology_seqkit/seqkit-v2.10.1/v2.10.1.mod) = e2c10d5a885a4ab21cc40f5665d8501580c40e8f136add7ee8b6d6f1e9395ca2
+SIZE (go/biology_seqkit/seqkit-v2.10.1/v2.10.1.mod) = 2516
+SHA256 (go/biology_seqkit/seqkit-v2.10.1/v2.10.1.zip) = 22d1ed2e8ac5a878948b832f52519cfa3e51f4b23ccec0ccbdc97e5e71a9ae81
+SIZE (go/biology_seqkit/seqkit-v2.10.1/v2.10.1.zip) = 34096290
+SHA256 (go/biology_seqkit/seqkit-v2.10.1/golang-sys-v0.6.0_GH0.tar.gz) = b4f6d17c7a128f76169964b437cb66b3f2dbf9a33361928ec19dfecf7b03fc54
+SIZE (go/biology_seqkit/seqkit-v2.10.1/golang-sys-v0.6.0_GH0.tar.gz) = 1434234