diff --git a/science/samtools/Portfile b/science/samtools/Portfile
index 34154356b1e..4ad111c3e31 100644
--- a/science/samtools/Portfile
+++ b/science/samtools/Portfile
@@ -7,11 +7,11 @@ PortGroup               legacysupport 1.1
 # strnlen
 legacysupport.newest_darwin_requires_legacy 10
 
-github.setup            samtools samtools 1.22.1
+github.setup            samtools samtools 1.23.1
 revision                0
-checksums               rmd160  c0f25de2226e2ed29b04c199793a65b25cd3f0e3 \
-                        sha256  02aa5cd0ba52e06c2080054e059d7d77a885dfe9717c31cd89dfe7a4047eda0e \
-                        size    9269357
+checksums               rmd160  d2fd92f08b59a1404894dfbd036abcbb5253fbbc \
+                        sha256  32266198a4bc6a6df395d8526688c9697d9c8e472f888c749fdde2e08ea88dd2 \
+                        size    9468164
 
 categories              science
 maintainers             {ryandesign @ryandesign} openmaintainer