diff --git a/samples/net/cloud/google_iot_mqtt/prj.conf b/samples/net/cloud/google_iot_mqtt/prj.conf
index 1bc427187b..205e359eb9 100644
--- a/samples/net/cloud/google_iot_mqtt/prj.conf
+++ b/samples/net/cloud/google_iot_mqtt/prj.conf
@@ -38,8 +38,6 @@ CONFIG_MAIN_STACK_SIZE=4096
 
 CONFIG_MBEDTLS_SSL_MAX_CONTENT_LEN=16384
 
-CONFIG_DEBUG_OPTIMIZATIONS=y
-
 CONFIG_PTHREAD_IPC=n
 CONFIG_NET_SOCKETS=y
 CONFIG_MQTT_LIB=y
diff --git a/tests/subsys/jwt/prj.conf b/tests/subsys/jwt/prj.conf
index 76dc7d28f9..673c5c2556 100644
--- a/tests/subsys/jwt/prj.conf
+++ b/tests/subsys/jwt/prj.conf
@@ -13,9 +13,6 @@ CONFIG_MBEDTLS_HEAP_SIZE=32700
 CONFIG_MBEDTLS_USER_CONFIG_ENABLE=y
 CONFIG_MBEDTLS_USER_CONFIG_FILE="user-tls.conf"
 
-CONFIG_DEBUG_OPTIMIZATIONS=y
-
 CONFIG_NET_SOCKETS=y
 
 CONFIG_NEWLIB_LIBC=y
-
diff --git a/tests/subsys/storage/stream/stream_flash/prj.conf b/tests/subsys/storage/stream/stream_flash/prj.conf
index 46211db136..6e5811b8fd 100644
--- a/tests/subsys/storage/stream/stream_flash/prj.conf
+++ b/tests/subsys/storage/stream/stream_flash/prj.conf
@@ -10,7 +10,6 @@ CONFIG_FLASH_PAGE_LAYOUT=y
 CONFIG_FLASH_MAP=y
 CONFIG_NVS=y
 CONFIG_SETTINGS=y
-CONFIG_DEBUG_OPTIMIZATIONS=y
 
 CONFIG_STREAM_FLASH=y
 CONFIG_STREAM_FLASH_ERASE=y